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. Author manuscript; available in PMC: 2018 Oct 10.
Published in final edited form as: J Control Release. 2017 Mar 27;263:18–28. doi: 10.1016/j.jconrel.2017.03.384

Fig. 6.

Fig. 6

Both efficacy and cancer-specificity of 536 25 w/w NPs were preserved in co-culture of HCC (Huh-7) and hepatocytes (THLE-3). A. Microscopy images and B. Flow cytometry gating of a 536 25 w/w NP-treated co-culture representative 48 hours after eGFP transfection. Microscopy images show: merged eGFP and RFP channels (top left), eGFP channel only (top right), bright-field only (bottom left), and RFP channel only (bottom right). C. Percentage of GFP positive cells among Huh-7 (RFP positive) and THLE-3 (RFP negative) by flow cytometry analysis. Co-cultures were plated and transfected in triplicate. * P < 0.05