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. Author manuscript; available in PMC: 2018 Nov 4.
Published in final edited form as: Biochem Biophys Res Commun. 2017 Sep 1;493(1):598–603. doi: 10.1016/j.bbrc.2017.08.146

Figure 2. Increased infiltration of MDSCs was accompanied by up-regulated gene expression of KLF4 and TSLP in the lung upon asthmatic challenge.

Figure 2

A. Mouse bronchoaveolar lavage fluids (BALF) were collected in control or OVA challenged mice, followed by cytokine measurement by ELISA. B. Single cells from lung tissues upon control or OVA challenge were purified, followed by flow cytometric analysis of MDSCs in the lung using CD11b and Ly6G antibodies. C. From the control and OVA challeged mice, total RNA from the lung tissues were extracted, cDNA were made and expression of KLF4 and FSP-1 in the lung were analysed by quantitative RT-PCR. * p<0.05 between control and treatment groups, n=3.