Immune sera were collected and pooled after immunization (n=5) with N1 NA (A/Cal), N2 NA (A/Wis), M2e5x VLP, split vaccine (A/PR8, A/Cal), or inactivated A/Phil H3N2 virus. NA inhibition assays were performed against different strains of influenza viruses: (A) A/Cal H1N1, (B) rgH5N1, (C) A/Wis H3N2, or (D) A/Phil H3N2. (E) HI titers against A/Cal H1N1, A/PR8 H1N1, rgH5N1, and A/Phil H3N2 were determined from immune sera of split vaccines (A/PR8 H1N1 and A/Cal H1N1).