Table 1.
Description of protein names, standard abbreviations, conventional zebrafish names (https://zfin.org) and function of endocannabinoid related genes investigated in this study.
Protein Name | Standard Abbreviation | ZFIN Gene Name | Function |
---|---|---|---|
cannabinoid receptor 1 | CB1 | cnr1 | G-protein coupled receptor located primarily in the CNS; activated by endogenous and exogenous cannabinoids |
cannabinoid receptor 2 | CB2 | cnr2 | G-protein coupled receptor located primarily in peripheral organs of the immune system and in the brain; activated by endogenous and exogenous cannabinoids |
G protein-coupled receptor 55a | GPR55A | gpr55a | G- protein coupled receptor widely expressed in the brain; recently found to be activated by endogenous and exogenous cannabinoids; its activation leads to stimulation of rhoA, cdc4 and rac1 |
cannabinoid receptor interacting protein 1a | CRIP1A | cnrip1a | CB1 receptor interacting protein that interacts with the distance C-terminus of CB1 altering/modulating CB1 interactions with G-protein |
monoglyceride lipase | MGLL (MAGL) | mgll | member of the serine hydrolases superfamily; it catalyzes the hydrolysis of 2-AG to AA and Glycerol |
αβ-hydrolase domain containing 6b | ABHD6B | abhd6b | member of the serine hydrolases superfamily; itcatalizes the hydrolysis of 2-AG to AA and Glycerol |
αβ-hydrolase domain containing 12 | ABHD12 | abhd12 | member of the serine hydrolases superfamily; it catalyzes the hydrolysis of 2-AG to AA and Glycerol in the CNS |
diacylglycerol lipase, alpha | DAGLα | dagla | diacylglycerol lipase; it catalyzes the hydrolysis of diacylglycerol (DAG) to the most abundantendocannabinoid 2-AG |
diacylglycerol lipase, beta | DAGLβ | daglb | diacylglycerol lipase; it catalyzes the hydrolysis of diacylglycerol (DAG) to the most abundant endocannabinoid 2-AG |
fatty acid amide hydrolase | FAAH | faah | fatty acid amide hydrolase with a single N-terminal transmembrane domain; principal catabolic enzyme for a class of lipids known as fatty acid amides (FAAs) of which AEA belongs to |
fatty acid amide hydrolase 2a | FAAH2A | faah2a | fatty acid amide hydrolase; it degrades bioactive fatty acid amides, including AEA (AEA = arachidonic acid + ethanolamine) |
N-acyl phosphatidylethanolamine phospholipase D | NAPE-PLD | napepld | member of the metallo-beta-lactamase family with phosphodiesterase activity; it releases NAE from NAPE to for AEA |
αβ-hydrolase domain containing 4 | ABHD4 | abhd4 | hydrolase that acts on either NAPE or lyso-NAPE to generate the glycerophospho-arachidonoyl ethanolamide which is subsequently cleavaged to give AEA |
glycerophosphodiester phosphodiesterase 1 | GDE1 (MIR16) | gde1 | phosphodiesterase with glycerophospho-NAE phosphodiesterase activity; ithydrolyses the phosphodiesterase bond of GP-NArE to release free AEA |
prostaglandin-endoperoxide synthase 2 a | PTGS2A (COX-2; COX-2A) | ptgs2a | cycloxigenase involved in AEA (and 2-AG?) metabolism |
N-acylsphingosine amidohydrolase (acid ceramidase) 1a | NAAA1A (ASAH1A) | asah1a | member of the choloylglycine hydrolase family with similar structure to faah. Unlike faah, naaa1a operates in acidic environments (pH 4.5) |
protein tyrosine phosphatase, non-receptor type 22 | PTPN22 | ptpn22 | protein tyrosine phosphase highly expressed in the immune system; it dephosphorylates pAEA to yield AEA |
peroxisome proliferator-activated receptor alpha b | PPARαβ | pparab | nuclear receptor transcription factor protein suggested as a binding target of endocannabinoids |
peroxisome proliferator-activated receptor gamma | PPARγ (ARF6) | pparg | nuclear receptor transcription factors protein suggested as a binding target of endocannabinoids |