TABLE 1.
Oligonucleotide primers used for qPCR and amplicon size
| Gene | Primer sequence, 5′–3′ | Size, bp | |
|---|---|---|---|
| Forward | Reverse | ||
| X. tropicalis | |||
| TH/bZIP | CCAAGGGAAACGGGTGGCTT | GTGCCACCTCTGCGGAAAGT | 172 |
| ST3 | GGTTATGTGTGGCGCCTTCG | AATGGGAAAGGGCCCAGAGG | 192 |
| Klf9 | GGCACAGGTGTCCTTATGCT | AAGGGCGTTCACCTGTATGG | 93 |
| X. laevis | |||
| TH/bZIP | CCACCTCCACAGAATCAGCAG | CAAGCAGAGAACGAGCAAGG | 91 |
| ST3 | GGGGGTTCCATGAACCGTGA | GAGCTCACTCAGCCCCTCTG | 147 |
| Klf9 | GCCCCAGTCAGGTCAACCAAT | CTTAAGGCAGTCAGGCCACG | 150 |
| X. tropicalis and X. laevis | |||
| EF1α | CGGAACTACCCTGCTGGAAG | GGCAAAGGTAACCACCATGC | 172 |