Figure 6.

BMPs promote HUVEC chemotaxis. A) HUVEC single-cell chemotaxis analysis showing the tracks of 35-40 individual cells upon side-directed treatment with 10 nM BMP2, 10 nM BMP6 or 2 nM VEGF for 16 h. A representative of 3 independent experiments is shown. B) Markov committor analysis of collective chemotaxis upon side-directed stimulation with indicated growth factors within 4 h. All HUVECs per optical field were tracked and analyzed using Markov state models. The red isoline separates the two zones in which cells are more likely to migrate upward or downward according to committor probabilities. Lighter area: higher chance of upward migration. Darker area: higher chance of downward migration. Shaded triangles mark the direction of the growth factor gradient.