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. 2017 Jul 7;31(11):4734–4744. doi: 10.1096/fj.201700197RR

Figure 2.

Figure 2.

Overexpression of the Gβ5-RGS7 complex promotes coupling of M3R to insulin secretion in MIN6 cells. A) MIN6 cells were transfected with plasmids encoding Gβ5 and RGS7 or lacZ and stimulated with the indicated concentrations of Oxo-M in the presence of 16.7 mM glucose. Insulin in the supernatants was measured by ELISA. Data are means ± sd from 3 independent transfections, each performed in triplicate. B) Transfected MIN6 cells were incubated with 100 μM Oxo-M or 0.1 μM GLP-1, 100 μM ADP, 0.1 μM AVP, all added in the presence of 16.7 mM glucose. For GLP-1 and AVP, there was a significant increase in insulin secretion compared to control; ADP did not cause significant change. C) The Gβ5−/− MIN6 cells (clone #1) or control CRISPR cells (Gβ5 +/+) were transfected with Gβ5 and RGS7 or control (LZ) plasmid, and then stimulated with 16.7 mM glucose or 16.7 mM glucose plus 100 μM Oxo-M (n = 3). Data are means ± sd. ***P < 0.001; **P < 0.01; *P < 0.05.