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. 2017 Jul 28;31(11):4959–4970. doi: 10.1096/fj.201700093R

Figure 6.

Figure 6.

NRF1 deficiency leads to reduced expression of germ cell–specific genes. A) Real-time RT-PCR analyses on the testes with conditional deletion of Nrf1 in germ cells at P5, compared to their wild-type littermates. Data are the mean of relative expression from 6 replicates ± 1 sem. **P < 0.01, *P < 0.05, ***P < 0.001. B) IHF of ASZ1 and TRA98 in testes from wild-type mice or mice with Nrf1 conditional deletion. Scale bar, 50 μm. C) IHF of germ cell–related proteins (DDX4, Lin28a, and DAZL) and HSP60 (a mitochondrial protein) in testes from wild-type mice or mice with Nrf1 conditional deletion. Scale bar, 10 μm. D) Protein levels of NRF1 target genes and genes involved mitochondrial activities measured by Western blots on the testes. E) Bisulfate sequencing of the Asz1 promoter from wild-type control and mice with Nrf1 deletion at P8. White circles, unmethylated CpGs; black circles, methylated CpGs.