Figure 6.

SHP-2 regulates neutrophil extravasation in vivo. Adult (age 8–10 wk) C57BL/6 mice were injected with control (Ctrl) or SHP-2 siRNA in liposomes. At 44 h after injection, mice were injected with LPS (8 mg/kg i.p.), and later lung tissue and BAL fluids were collected at 0, 2, 4, and 8 h. A) Down-regulation of SHP-2 by siRNA silencing was estimated by Western blot. B–D) Lung tissue MPO activity (B), BAL MPO activity (C), and protein contents in BAL were determined (D; n = 3 mice for each group). The blots of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were derived from the same samples. Data are presented as means ± sem of 3 independent experiments, and data analysis was performed by Bonferroni post-tests after 2-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001.