Figure 7.

LPS-induced degradation of VE-cadherin in mouse lungs after SHP-2 down-regulation. Adult (age 8–10 wk) C57BL/6 mice were treated as in Fig. 6. Lungs were collected and lysed for total protein level by Western blot. Expression of ICAM-1 (A), phosphorylation levels of Src at Y419 (human; B), and protein levels of VE-cadherin (C) were determined by Western blot. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) served as loading control and the blots of GAPDH were derived from the same samples (n = 3 mice for each group). Ctrl, control. Data are presented as means ± sem of 3 independent experiments and data analysis was performed by Bonferroni post-tests after 2-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.