Figure 3.

Impaired angiogenic response to ischemic injury in GR^LysMCre mice. A) Immunohistochemical staining (CD31, brown; α-SMA, green) showing capillaries, coated vessels, and myofibroblasts in the healing myocardium of GR^flox and GR^LysMCre mice 7 d after MI. Neovasculature in GR^LysMCre mice seems to be immature and collapsed, as indicated by a lack of open lumen in the CD31⁺ vessels. Myofibroblasts appear considerably larger compared with those in GR^flox mice (enlarged insets). B) Capillary density quantification in GR^flox and GR^LysMCre mice 7 d after MI. Scale bars, 100 µm; enlarged insets, 50 µm. Means ± sem (n = 6). *P < 0.05 vs. GR^flox.