Figure 4.

Deletion of GR affects the differentiation/maturation of monocyte-derived macrophages in infarcted myocardium. A, B) Cells were sorted from GR^flox (A) and GR^LysMCre (B) infarcts 3 d after coronary artery ligation. Monocyte-derived macrophages were identified as CD45⁺/CD11b⁺/Ly6G⁻/F4/80⁺ cells, then further stratified by Ly6C and major histocompatibility complex class II expression. C) Cytospin preparation of sorted F4/80⁺/Ly6C^high and F4/80⁺/Ly6C^low cells. Scale bars, 50 µm. D) FACS-based quantification of cells per milligram tissue. Means ± sem (n = 5). *P < 0.05 vs. GR^flox.