Figure 6.
E-selectin-dependent rolling promotes stationary Pa03c cell adhesion to HA. All cells interacting with HA were tracked immediately after their initial binding interaction and classified as stationary or transiently adherent with a cutoff threshold of 2 s. A) The percentage of stationary adherent cells on HA. Data are means ± 95% confidence intervals. B) The multibond model was fitted to the experimental data (points) and optimized for both AcMrMlKon and n bonds, with the solid lines representing the model prediction for Pa03c cell adhesion to HA. Data are means ± 95% confidence intervals. C) The AcMrMlKon (lumped binding affinity) to HA was determined based on the experimental data for cells, which first rolled on E-selectin before passing over a 30- or 60-µm gap region, as well as nonrolling cells.