Expression of nNOS in the small intestine of transgenic mice with age and the co-localization of nNOS and α-syn. A Expression of nNOS in different sections (proximal, middle, and distal segments) of the intestine in transgenic mice. B α-Syn and nNOS expressed in transgenic mice and the co-localization at 3, 6, and 24 months. C Co-localization of nNOS and α-syn, and the nucleus of an enteric neuron at 24 months. Scale bars 200 μm in A, B; 10 μm in C.