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. 2017 Oct 11;7:12991. doi: 10.1038/s41598-017-13408-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of MEA cultures. (a) MEA were incubated with a polyclonal anti-GFAP antibody, and GFAP immunoreactivity was detected using the avidin-biotin complex for amplifying the target antigen signal, as indicated in materials and methods. (b) Negative control (without anti-GFAP antibody). (c) MEA were incubated with a polyclonal anti-vimentin antibody. (d) MEF were incubated with the monoclonal anti-GFAP antibody. In both cases, antigen signal was amplified as indicated above.