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. 2017 Oct 11;7:12944. doi: 10.1038/s41598-017-13325-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The images of the living HeLa cells co-incubated with the probe Na-H ₂ S-ER (5 μM), Na₂S (50 μM), and ER-Tracker TM Red. (a) The fluorescence image of the green channel; (b) The fluorescence image of the red channel; (c) The merged image of (a and b,d) Intensity scatter plot of the green and red channels. (e) Intensity profile of linear region of interest across in the HeLa cells co-stained with ER-Tracker Red and green channel of Na-H ₂ S-ER. Scale bar: 20 μm.