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. 2017 Oct 11;7:12981. doi: 10.1038/s41598-017-12819-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Isogenic MDCKI cell lines for WT-NCC and K706R, K828R and K909R mutants show tetracycline inducible NCC expression. Representative immunoblots of NCC and actin in protein samples isolated from various MDCKI cell lines grown on plastic. Following tetracycline (10 µg/ml) induction NCC is observed as a core non-glycosylated band around 100 kDa and a protein smear at 130 kDa corresponding to the glycosylated monomer. Samples are not run on the same blot, hence a direct comparison of expression levels is not possible.