Table 1.

Ubiquitylation sites in NCC identified by Ubiscan in this study or from previously published studies^13,14.

AA position human and (mouse) NCC	Ubiscan of mouse kidney	Human urinary exosomes	Wagner et al., 2012	Conserved (mouse and human)	Location	Examined in this study
81 (79)	+		+	+	amino terminus	+
94 (92)		+		+	amino terminus
128 (126)	+	+	+	+	amino terminus	+
199 (197)			+	+	Intracellular loop 1	+
706 (704)			+	+	carboxyl terminus	+
784 (782)		+		−	carboxyl terminus
801 (799)		+		−	carboxyl terminus
814 (795)		+		−	carboxyl terminus
828 (809)	+		+	+	carboxyl terminus	+
877 (858)			+	+	carboxyl terminus	+
885 (866)	+			+	carboxyl terminus	+
902 (883)	+		+	−	carboxyl terminus
909 (890)	+	+	+	+	carboxyl terminus	+
925 (906)			+	+	carboxyl terminus	+
940 (921)			+	+	carboxyl terminus	+
948 (929)	+		+	+	carboxyl terminus

The location of the sites relative to NCC topology and the conserved sites examined further in this study are highlighted.