Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 11;7:12970. doi: 10.1038/s41598-017-13438-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Lateral diffusion of lipids is not affected by cytoskeletons. The cell membrane was stained with Polaric, a similar fluorescent lipid analogue to CellMask Orange. (A) Typical fluorescence images when a small region of the cell membrane was photobleached (0 sec). (B) The fluorescence intensity profiles in the white rectangle in panel A at each time (−0.9, 0.0, 1.8, and 8.0 sec). Note that the fluorescence recovered by a simple lateral diffusion of the lipids. (C) A time course of fluorescence recovery in the bleached circles. (D) A comparison of the diffusion coefficient in the presence of inhibitors (latrunculin B, thiabendazole, blebbistatin, and sodium azide), indicating that all these inhibitors do not affect the lateral diffusion of the membrane lipids (n = 20 each). Data are presented as mean ± SD and analyzed by one way ANOVA with Tukey’s multiple comparison test. ns, not significant. Bar, 10 µm.