Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 11;7:12984. doi: 10.1038/s41598-017-13326-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Live/Dead Assay was done to test the toxicity of HCCS-PDA and HCCS, with viable rMSCs staining as green by Calcein-AM and dead rMSCs staining as red by EtD-1 (A), scale: 500 μm. The rMSC proliferation on the HCCS-PDA and HCCS coated dishes was assessed by MTS on 1, 3, 5, and 7 days of culturing, with a non-coated culture dish as the control (B), scale: 50 μm. rMSC adhesion assay on the coated culture dish with HCCS-PDA and HCCS was performed after 2, 6, 16 and 24 hours by counting the number of attached cells. Cell morphology was observed after immunostaining with focal adhesion assay kit (C), scale: 10 μm. Mineral nodule formation after Alizarin Red S staining and CPC quantification was assessed. Osteogenic differentiation was performed using 50% reduced osteogenic factors in the media. Enhanced mineralization was observed in the rMSCs cultured with growth media for 28 days on HCCS-PDA, with the formation of small minerals evident (D), GM: growth media and OM: osteogenic media. Extracted solution by 1% CPC was measured at an absorbance of 570 nm (n = 5, *p < 0.05 vs. in osteogenic media on HCCS, and ^# p < 0.05 between in growth media without DA and with DA). Image J analysis of the mineral coverage of the culture dish. Both CPC and Image J analysis yielded similar results.