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. 2017 Oct 11;7:12996. doi: 10.1038/s41598-017-13522-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of lentivirally transfected mouse fetus-NSPCs and mouse iPSC-NSPCs in vitro. (A) Phase contrast, (B) fluorescence, and (C) luminescence images. (D,E) Immunostained images of neurospheres derived from mouse fetus-NSPCs and miPSC-NSPCs (after in vitro differentiation with antibodies against glial fibrillary acidic protein (GFAP), CNPase, βIII tubulin, GFP, MHC class I, MHC class II, and Hoechst). Bioluminescence imaging was conducted in various numbers of fetus-NSPCs and iPSC-NSPCs (0, 1 × 10⁵, 2.5 × 10⁵, 5 × 10⁵, and 1 × 10⁶ cells per well). A correlation between luminescence and the number of cells was confirmed. Fetus-NSPCs and iPSC-NSPCs differentiated into β-III tubulin + neurons, GFAP + astrocytes, and CNPase + oligodendrocytes in vitro (D). The expression of MHC class I or II was not observed (E). Scale bars = 1,000 µm in (A) and 100 µm in (B), (D), and (E).