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. 2017 Oct 11;7:12996. doi: 10.1038/s41598-017-13522-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fetus-NSPCs and 2A4F iPSC-NSPCs triggered allogeneic PBMC, but not syngeneic PBMC, proliferation in vitro. Fetus-NSPCs and 2A4F iPSC-NSPCs were irradiated and co-cultured with syngeneic mouse (C57BL6/J) PBMCs and allogeneic mouse (BALB/cA) PBMCs (pre-immunized and post-immunized) at a ratio of 1:10 with or without IFNγ and anti-TGFβ receptor antibodies for 5 days (n = 3 independent experiments). (A,C) CPM measured in MLR assays without (A) or with (C) the anti-TGFβ antibody. (B,D) The SI was calculated as the ratio of the CPM in each MLR assay relative to the CPM in a syngeneic MLR assay. Values are shown as the mean ± SEM. *P < 0.05 and **P < 0.005, unpaired two-tailed Student’s t-test or one-way ANOVA followed by the Tukey–Kramer test. ns = not significant.