Figure 5.

Histological examination of grafted syngeneic and MHC-mismatched allogeneic mice spinal cords. Fetus-NSPCs and iPSC-NSPCs (2A3F and 2A4F) transplanted into allogeneic hosts were stained with hematoxylin-eosin (A) and immunostained with antibodies against GFP, βIII tubulin, CNPase, MHC class I, MHC class II, CD163, TGFβ and Hoechst (B–F). All cell populations differentiated into three neural lineages. MHC expression was observed in a very small proportion of these cells after transplantation. Inflammatory cell infiltration was more obvious in allogeneic transplantation than syngeneic transplantation. Many of the infiltrating CD11b + macrophages were positive for CD163 and TGFβ. (G) GFP, CD4, CD8, CD11b, and CD335 staining and quantified of inflammatory cells in mice with surviving syngeneic or allogeneic grafts. CD4 + and CD11 + cells infiltrated the allogeneic host spinal cord significantly more than syngeneic host spinal cord. Values are shown as the mean ± SEM. *P < 0.05, unpaired two-tailed Student’s t-test and one-way ANOVA followed by the Tukey–Kramer test. ns, not significant. Scale bars = 500 µm in (A) and (B) and 100 µm in (C–G).