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. 2017 Oct 11;7:12996. doi: 10.1038/s41598-017-13522-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histological examination of cells after transplantation into intact and injured spinal cords of BALB/cA mice. Fetus-NSPCs and 2A4F iPSC-NSPCs were transplanted into intact spinal cord, injured spinal cord, and injured spinal cord in the presence of FK506. Inflammatory cells (CD4, CD8, CD11b, and CD335) surrounding the surviving grafts were stained (A) and quantified (B). Values are shown as the mean ± SEM. *P < 0.005, unpaired two-tailed Student’s t-test and one-way ANOVA followed by the Tukey–Kramer test. ns, not significant. Scale bars in (A) = 100 µm.