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. 2017 Oct 11;7:12974. doi: 10.1038/s41598-017-13494-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PTPN1 regulates JNK-related cell death response. (A) Flag-PTPN1 or Flag-PTPN1 D181A was expressed in 293 T cells. Control cells were transfected with an empty vector. Upon TNFα-stimulation, protein expression and JNK phosphorylation were each examined by immunoblotting. (B) JNK activity was assessed using a luciferase reporter gene assay. The data are presented as the mean ± SD (repeats were performed in triplicate). (**p < 0.005) (C) Flag-PTPN1 or FLANG-PTPN1 D181A was expressed in p65-deficient MEF cells. After TNFα-stimulation (7 h), dead cells were visualized with PI staining (7 µg/ml) and observed via microscopy. (D) The relative cell death ratio was plotted ( ± SD, n = 3). (*p < 0.05).