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. 2017 Oct 11;7:12980. doi: 10.1038/s41598-017-12734-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ALFY is upregulated during ATRA-induced differentiation of AML cells. (a–c) NB4, NB4-R2 and HT93 APL cells as well as the non-APL HL60 cell line and its ATRA-resistant clone HL60-R2 were treated with 1 µM ATRA for 4 and 6 days. ALFY mRNA levels were determined by qPCR. Raw Ct values were normalized to HMBS and to the untreated control of day 4. Standard deviations are shown for at least three independent experiments. (d–e) NB4 cells were transduced with two shRNAs (#1 and #2) targeting different regions of the PU.1 or CEBPA mRNA or a non-targeting control (CTRL). After treatment with 1 µM of ATRA for 4 days ALFY mRNA levels were determined by qPCR and normalized as described in A.