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. 2017 Oct 11;7:12963. doi: 10.1038/s41598-017-13526-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of Chit1 and AMCase mRNAs in pig tissues. Quantification of (a) Chit1 and (b) AMCase mRNAs in ten major pig tissues. Both chitinases were quantified by qPCR using the standard DNA. All values obtained were expressed as molecules per 10 ng of total RNA. (c) Ratios of AMCase to Chit1. All mRNA copy numbers were derived based on the same standard dilutions. The upper panels indicate the actual number, whereas the lower panel shows each value on logarithmic scale. Each reaction was performed in triplicate. **p < 0.01. P-values were determined using Student’s t-test.