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Purification of AMCase from pig stomach.
| Purification step | Total activity (mU) | Total Protein (mg) | Specific activity (mU/mg) | Yield (%) |
|---|---|---|---|---|
| Total soluble fraction | 542.9 ± 3.3 | 20.2 ± 1.5 | 26.8 ± 0.2 | 100 |
| Chitin beads Flow through | 61.5 ± 2.4 | 12.2 ± 2.1 | 5.0 ± 0.2 | 11.3 |
| Purified enzyme | 49.6 ± 2.1 | 0.023 ± 0.006 | 2174.0 ± 90.4 | 10.3 |
The purified protein was prepared from 1 G of stomach tissue as described in the Methods.
