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. 2017 Jun 13;95(9):744–752. doi: 10.1038/icb.2017.43

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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SHM levels and IGHG subclass distributions in memory B cell subsets. (a) The frequencies of IgG⁺ B cells that express CD27 increase with age. (b) IGHG subclass distributions of CD27⁺ (eight donors) and CD27⁻ IgG⁺ memory B-cell subsets (six donors). (c) SHM frequencies in IGHV of IGHG transcripts from memory B-cell subsets. Numbers of transcripts are indicated in brackets. Statistical analysis of SHM was done with the Kruskal–Wallis test and Dunn’s multiple comparison test and of CSR with the χ² test; *P<0.05; **P<0.01; ***P<0.001.