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. 2017 Jul 25;95(9):775–788. doi: 10.1038/icb.2017.50

Figure 1.

Figure 1

LRBA deficiency decreases CTLA-4 on CD4 effector/memory and Tregs. Flow cytometric analysis of spleen cells from age- and sex-matched Lrba−/− (blue) or WT (red) mice at 6 (n=6) or 26 weeks (n=4) of age. (a–c) CTLA-4 expression on CD4 memory/effector T cells. (a) Representative flow cytometry plots of permeabilised CD4+ FOXP3− cells from 6-week-old mice showing % CTLA-4+ CD44hi cells and histograms of CTLA-4 in the CD4+ CD44hi CTLA-4+ population in 6- and 26-week- old mice. Lrba+/− are indicated in pale blue. Graphs show (b) the number of CTLA-4+ CD4+ CD44hi FOXP3− cells and (c) CTLA-4 mean fluorescence intensity (MFI) in individual mice and arithmetic mean for each genotype and age group. (d–g) CTLA-4 expression on CD4+ Treg cells. (d) Representative plots of permeabilised CD4+ cells showing the % FOXP3+ Treg cells in 6-week-old mice. Representative Treg histograms and MFI for: (e) CTLA-4; (f) FOXP3; (g) CD25. Statistical analysis was carried out using t-test: *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001. Data are representative of one experiment.