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. 2017 Apr 25;2(2):113–120. doi: 10.1016/j.synbio.2017.04.001

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 1 — Schematic representation of the construction of the expression vectors in this work. (A) The inducible expression vectors, pAOαMH-Kla and pAOαMH-Aor, carrying β-galactosidase gene from Aspergillus oryzae and Kluyveromyces lactis, respectively. (B) The constitutive expression vectors, pGAPα-Aor, pSDHZαH-Aor and pTef1ZαH-Aor with P_GAP, P_SDH, and P_TEF1 as promoters. pGAPα-Aor contains no HIS4 gene. (C) The chaperone co-expression vectors, pGAPZ-PDI, pGAPZ-KAR and pGAPZ-SSO carrying PD1, KAR2 and SSO1, respectively.