Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 7;8(9):e3046. doi: 10.1038/cddis.2017.382

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

ZNF503 promotes RPE differentiation, and inhibits cell proliferation and migration. (a and b) ZNF503 expression was elevated in ARPE19 cells transfected with Ac-ZNF503-AS1 when compared to cells transfected with empty vector (a), and was reduced in cells transfected with ZNF503-AS1 siRNA when compared with cells transfected with scramble siRNA (b). (c) ZNF503 expression was remarkably decreased in ARPE19 cells transfected with ZNF503 siRNA. (d) In ARPE19 cells transfected with ZNF503 siRNA, mRNA expressions of RPE relevant markers, including RPE65, RLBP1, LRAT, KRT18, and CTNNB1, were upregulated. (e and f) Immunoblotting indicated that protein expressions of Mertk, cytokeratin-18, and β-catenin were increased in ARPE19 cells transfected with ZNF503 siRNA compared to cells transfected with scramble siRNA. (g and h) Immunofluorescent staining suggested that ZO-1 (g) and β-catenin (h) expressions were elevated in ARPE19 cells transfected with ZNF503 siRNA when compared to cells transfected with scramble siRNA. No obvious mislocalization of proteins was identified in all transfected groups