Figure 6.

Elevated LGR5 enhances migration, invasion and colony formation ability of cervical cancer cells in vitro. (a, b) The overexpression of LGR5 enhances migration in wound healing assays. Columns and error bars represent means±S.D. from one experiment of three independent experiments (n=6 scratches per cell type and time point in each experiment). Scale bars, 800 μm. (c) Cell migration and invasion was measured in Transwell chambers. Cells were counted with a microscope in nine random high-power fields. Migration and invasion ability was significantly increased in LGR5-overexpressing SiHa and HeLa cells. (d) Expression levels of EMT-related proteins in the LGR5-overexpressing and LGR5-knockdown HeLa and SiHa are demonstrated by western blotting. (e, f) Each 1000 cells were cultured in soft agar plates for 2 weeks. The number of colonies was assessed using crystal violet staining. Columns and error bars represent means±S.D. of three independent experiments using triplicate measurements in each experiment. *P<0.05; **P<0.01; ***P<0.001