Figure 6.

ANGPTL4 plays an important role in rosiglitazone-induced angiogenesis. (a–c) HUVECs transfected with si-Con or si-ANGPTL4 were stimulated with 1 μM rosiglitazone. Control cells were treated with 1 μM rosiglitazone or 100 nM rhANGPTL4. Tube formation assay was performed in these cells, and then the mRNA and protein levels of VEGF and its secretion were evaluated through western blot, qRT-PCR and ELISA. The data are shown as the means±S.E.M. *P<0.05, **P<0.01 and ***P<0.001 relative to control or si-Con. (d) The expression of PPARγ, ANGPTL4, VEGF and CD31 in placental tissues was examined by IHC in PE subjects (n=30) and normal controls (n=30). Representative images were captured at × 400 magnification