Figure 6.

I-Trp promotes ER stress-induced cell apoptosis via the MAPK pathway upon CCT-β : β-tubulin complex destruction. (a) Quantification of cellular JNK, p-JNK, ERK, pERK, p38, and p-p38 upon I-Trp treatment by western blot. Using the kinase inhibitors to test the pathway of cell apoptosis induced by I-Trp. Commercially available ERK inhibitor U0126 (b), JNK inhibitor SP600125 (c), and P38 inhibitor SB203580 (d) were used to inhibit the kinases, respectively, and the sub-G₀ cells were accessed using flow cytometer. Data are expressed as mean±S.D. ***P<0.001, significant difference as compared to control. ^#P<0.05, ^##P<0.01, and ^###P<0.001 significant difference as compared to I-Trp alone