Figure 1.

Endogenous SIRT7 expression and the construction of SIRT7-konckdown hBMSCs and lenti-control hBMSCs. (a–c) The endogenous expression of SIRT7 mRNA and protein were determined, respectively, by qPCR and western blotting analysis at days 0, 3, and 7 of osteogenic differentiation. (d) hBMSCs after lentiviral transfection and puromycin screening were observed under a normal microscope and a fluorescence microscope. (e–g) The mRNA and protein levels of SIRT7 were determined, respectively, by qPCR and western blotting analysis among the lenti-SIRT7, lenti-control group, and mock treated group. (h) The proliferation rate of hBMSCs was not significantly affected by SIRT7 knockdown. The mRNA and protein expression levels were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). All the data were confirmed by three repeated tests. The data are expressed as means±S.D., *P<0.05 versus the lenti-control group