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. 2017 Sep 7;8(9):e3042. doi: 10.1038/cddis.2017.429

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Immunofluorescence staining showed the protein levels of RUNX2, SIRT7, and β-catenin. (a) The level of RUNX2 protein (red) at day 3 of osteogenic differentiation. (b) The level of SIRT7 protein (red) at day 3 of osteogenic differentiation. (c) The level of β-catenin protein (red) at day 3 of osteogenic differentiation. The nuclei were counterstained with DAPI (4′,6-diamidino-2-phenylindole; blue). All the data were confirmed by three repeated tests. Scale bar=100 μm