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. 2017 Sep 7;8(9):e3042. doi: 10.1038/cddis.2017.429

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The knockdown of SIRT7 upregulated the Wnt/β-catenin signaling pathway during osteogenesis. (a) The expression of β-catenin mRNA was determined by qPCR at days 3 and 7 of osteogenic differentiation. (b) The expression of GSK3β mRNA. (c) The expression of Axin mRNA. (d–f) The expression of active β-catenin and total β-catenin proteins were determined by western blotting analysis at days 3 and 7 of osteogenic differentiation. All the data were confirmed by three repeated tests. Data were mean±S.D. *P<0.05 versus the lenti-control group