Figure 5.

The increased osteogenesis caused by SIRT7 konckdown could be rescued partially by the addition of a Wnt/β-catenin signaling inhibitor (DKK1). (a) The expression of β-catenin, GSK3β, and Axin mRNA in the lenti-control, lenti-control+DKK1, lenti-HSPA1A, and lenti-HSPA1A+DKK1 groups were determined by qPCR at days 3 of osteogenesis. (b) The expression of RUNX2, OSX, OPN, and COL1A1 mRNA at days 3 of osteogenesis. (c) The expression of RUNX2, COL1A1, active β-catenin, and total β-catenin proteins were determined by western blotting analysis at day 3 of osteogenesis. (d and e) ALP staining and activity at day 7 of osteogenesis. (f and g) ARS and quantitation at day 9 of osteogenesis. All the data were confirmed by three repeated tests. Data were mean±S.D. *P<0.05 versus the lenti-control group. #P<0.05 versus the lenti-SIRT7 group. Scale bar=200 μm