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. 2017 Sep 7;8(9):e3044. doi: 10.1038/cddis.2017.439

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Presence of fluorescence-tagged angiotensin receptors and fluorescent AII in nuclei. Colocalization (c,f and i) of the fluorescent nuclear marker Hoechst (a,d and g) with AT1-EGFP (b), AT2-YFP (e) or AII-Rhod (h). WB analysis of GFP-/YFP-tagged protein in MES 23.5 transfected (+) and not transfected (−) cells showing the specificity of the common anti-GFP/YFP antibody (j). Treatment of MES 23.5 dopaminergic neuron cell line with AII increased nuclear AT1-EGFP receptor protein but not nuclear AT2-YFP receptor protein relative to nuclei of control cells (k and l). Data are mean±S.E.M. *P<0.05 compared to control. Student’s t-test (n=3–4). AT2-YFP, AT2 tagged to YFP; AT1-EGFP, AT1 tagged to EGFP; AII-Rhod, rhodamine-fluorescent AII. Scale bar: 20 μm (a–i)