Figure 6.

Inhibition of JNK/c-jun and autophagy increases GA-induced ER stress in sarcoma cells. (a and b) After suppression of the JNK/c-jun cascade by SP600125 (10 μM, 1 h) pretreatment, HT1080 and HOS cells were treated with GA (40 μM) for 24 h. Protein expression levels were detected by western blot analysis. *P<0.05. (c and d) After suppression of the JNK by shRNA transfection, HT1080 and HOS cells were treated with GA (40 μM) for 24 h. Protein expression levels were detected by western blot analysis. *P<0.05. (e and f) HT1080 and HOS cells were treated with GA (40 μM) for 24 h with or without pretreatment of CQ (5 μM, 1 h). Western blot analysis was used to examine protein expression. *P<0.05. (g and h) HT1080 and HOS cells were treated with GA (40 μM) for 24 h with or without pretreatment of 3-MA (5 mM, 2 h). Western blot analysis was used to examine protein expression. *P<0.05