Figure 7.

Inhibition of autophagy or JNK enhances GA-induced cell proliferative inhibition and apoptosis in sarcoma cells. (a) 3-MA or bafilomycin A1 was pretreated to HOS and HT1080 cells for 6 h before incubating in GA (40 μM) for 24 h. Western blot analysis examined the protein expression level of Beclin-1, BCL-2, BCL-xl, survivin, cleaved-PARP, and LC3 conversion. (b) After pretreatment with 3-MA or bafilomycin A1 for 6 h, HOS and HT1080 cells were incubated in GA (40 μM) for 24 h. Then, MTT assay was used to test cell viability to calculate the growth index. (c) Cells in (b) were analyzed by flow cytometry. Histograms were shown for analyzed cells (n=3). *P<0.05. (d) After knockdown of JNK or Atg5 by siRNA, HOS and HT1080 cells were incubated with 40 μM of GA for 24 h. Western blot analysis examined the protein expression level of Atg5, BCL-2, BCL-xl, survivin, cleaved-PARP, and LC3 conversion. (e) After knockdown of JNK or Atg5 by siRNA, HOS and HT1080 cells were incubated in GA (40 μM) for 24 h. Then, MTT assay was used to test cell viability to calculate the growth index. (f) Cells in (e) were analyzed by flow cytometry. Histograms were shown for analyzed cells (n=3). *P<0.05