Figure 2.

RL71-induced cell death is mainly mediated by autophagy. MDA-MB-468 cells were transiently transfected with control siRNA, siRNA targeting ATG5 or ATG7 for 24 h, and then treated with various concentrations of RL71 for 48 h. (a) Knockdown of ATG5 or ATG7 was confirmed by western blot. **P<0.01 versus NC-siRNA controls. (b) Cell viability was determined by MTT assay. (c) MDA-MB-468 cells were pretreated with 3-MA or CQ for 2 h before treatment with various concentrations of RL71 for 24 h. Cell viability was determined by MTT assay. The data are the mean±S.D. of three independent experiments. *P<0.05, **P<0.01, ***P<0.005