Figure 7.
RL71 prevents tumor development in MDA-MB-231 orthotopic inoculation model. Female nude mice were inoculated with 2 × 106 MDA-MB-231 cells into the mammary fat pad. After 14 days, mice (n=8 in each group) were treated with olive oil, RL71 (2 or 4 mg/kg/day) or PTX (10 mg/kg/7 days) intraperitoneally for an additional 14 days. (a) The tumor growth curves were determined by measuring the tumor volumes. Left panel: representative images of the tumors at the end of the experiment. The data are the mean±S.D. of eight mice per group. *P<0.05, **P<0.01 versus the olive oil controls. (b) Tumors excised on day 15 were weighed. *P<0.05, **P<0.01 versus the olive oil controls. (c) The body weight was monitored during the experiment. (d) Liver and spleen excised on day 15 were weighed. (e) Representative images of lung section stained with H&E. The lung tissues were excised on day 15. Black arrow, metastatic foci in the lungs. Scale bar: 100 μm. (f) The proportion of mice with lung metastases in each group. (g) Protein levels of CHOP, caspase-3, p62 and LC3B in tumor samples. The tumor tissues were excised on day 15 and analyzed by western blot. *P<0.05, **P<0.01 versus the olive oil controls. (h) Tumor sections were assayed by TUNEL or (i) stained using p62 antibody. The tumor tissues were excised on day 15. The data shown are representative of three experiments. Scale bar: 100 μm