Figure 8.
RL71 prevents tumor development in SUM-1315 xenograft model. Female nude mice were inoculated with 5 × 106 SUM-1315 cells into the right flank. After 7 days, mice (n=8 in each group) were treated as described in Figure 7. The tumor tissues were excised on day 15 (a) The tumor growth curves. Left panel: representative images of the tumors at endpoint. The data are the mean±S.D. of eight mice per group. *P<0.05 versus the olive oil controls. (b) Tumor weight. *P<0.05, **P<0.01 versus the olive oil controls. (c) Body weight. (d) Weight of liver and spleen on day 15. (e) Survival curve of mice (n=6 mice per group). (f) Protein levels of CHOP, caspase-3, p62 and LC3B in tumor samples. *P<0.05, **P<0.01 versus the olive oil controls. (g) Tumor sections were assayed by TUNEL or (h) stained using LC3 antibody. The data shown are representative of three experiments. Scale bar: 100 μm