Figure 8.

GyrA^ΔCTD gyrase maintains lower levels of cleavage complexes on positively supercoiled DNA. Left: Levels of cleavage complexes generated by varying concentrations of GyrA^ΔCTD gyrase on positively supercoiled [(+)SC, red] or negatively supercoiled [(−)SC, blue] DNA in the absence of quinolones. Right: Levels of cleavage complexes generated by GyrA^ΔCTD gyrase on positively supercoiled [(+)SC, red] or negatively supercoiled [(−)SC, blue] DNA in the presence of moxifloxacin. Error bars represent the standard deviations for at least three independent experiments.