Figure 3.

Null dfmr1 neurons manifest dye injection defect without gap junctions. A, Representative images of anti-ShakB labeling in the GFI for the w¹¹¹⁸ genetic background (control; top) and dfmr1^50M-null mutant (bottom). White outline indicates GFI bend as labeled by injected TRITC signal. ShakB signal intensity is represented as a heat map. Arrows indicate ShakB punctae. Scale bar, 5 μm. B, Quantification of ShakB signal intensity in both genotypes, displayed as mean ± SEM. Control, n = 27; dfmr1, n = 26. C, Representative NB injections into the GFI (2 m KAc) for the shakB² single-mutant (left) and shakB²; dfmr1^50M double-mutant (right). Scale bar, 10 μm. D, Quantification of the injected dye levels in both genotypes, displayed as mean ± SEM. Control, n = 30; dfmr1, n = 31. Significance was determined from two-tailed unpaired t tests: **p < 0.01.