Skip to main content
. 2017 Oct 11;37(41):9844–9858. doi: 10.1523/JNEUROSCI.0723-17.2017

Figure 5.

Figure 5.

Dye injection defect is not related to multiple GFI neuron properties. A, Representative TRITC injections (ddH2O) showing the GFI descending axon for w1118 genetic background (control; left) and dfmr150M-null mutant (right). Scale bar, 5 μm. B, Quantification of the axon diameter in both genotypes, displayed as the mean ± SEM. Control, n = 15; dfmr1, n = 16. C, Representative NB dye injections into GFI for the w1118 genetic background (control; top) and the dfmr150M-null mutant (bottom) using only KAc-free dye solution (ddH2O). Scale bar, 20 μm. D, Quantification of the injected dye levels, displayed as mean ± SEM. Control, n = 25; dfmr1,n = 26. E, Representative NB injections (ddH2O) into GFI for w1118 genetic background (control; top two panels) and dfmr150M-null mutant (bottom two panels). After dye injection, samples were either immediately dissected (0m) or injected with TRITC for 5 min (5m) with positive current before dissection. Scale bar, 20 μm. F, Quantification of injected dye levels, displayed as the mean ± SEM. 0 m: control, n = 6; dfmr1, n = 6; 5 m: control, n = 6; dfmr1, n = 5. Significance determined from two-tailed unpaired t test (D) and unpaired ANOVA (F): *p < 0.05, ***p < 0.001.