Figure 1.

Preexposure of acute brain slice to Glut increases SIC emergence. A, Left diagram, Brain slices were cut and placed in control aCSF (gray dish) or in a Glut preexposure aCSF (black dish). Top middle trace (gray) from a VB TC neuron in a slice stored in control aCSF exhibiting a single SIC. Bottom (black) trace is a patch-clamp recording from a TC neuron in a slice preexposed to aCSF containing 200 μm Glut for 4 h, exhibiting a large number of SICs. Bar graph on the right illustrates SIC frequency from a number of experiments. White circles indicate values from individual recordings for the two conditions. B, Cumulative probability curves for SIC amplitude, charge, rise times, and decay times for control (gray plots) and >;2 h Pre-Glut treatment (black). C, Example traces from three different cells preexposed to Glut containing aCSF for the indicated times before recording. D, Plot from a number of experiments of SIC frequency against preexposure time for control aCSF (gray symbols) and Glut-containing aCSF (black symbols) fitted with regression lines (left) and variation of mean charge (Q, right) with pretreatment times for control (gray lines and symbols) and Glut (black lines and symbols) containing aCSF. E, Mean SIC frequency at different time points during recording for cells from the two groups showing that increased SIC frequency in Glut preexposed slices is sustained. All recordings were conducted in the presence of TTX.