FIG 2.
Charge-change mutations in BamA POTRA5 cause growth defects. Strains containing an arabinose-inducible copy of bamA (JCM320) as well as pZS21::bamAmut were grown to stationary phase with arabinose, 10-fold serial dilutions (top) were spotted onto LB medium with 25 μg/ml kanamycin or LB with 25 μg/ml kanamycin and 0.2% arabinose, and plates were incubated overnight at 37°C. (A) bamAD362R and bamAR366E are synthetically lethal with bamDR197L, while bamAE373K is synthetically lethal with bamD+. bamAK351E showed no growth defects. (B) bamAK351E is an intragenic suppressor of bamAE373K but not bamAD362R. (C) Both bamAD362R and bamAR366E show reciprocal incompatibility with bamAE373K.